Blastocyst Implantation Transfer
IVF proves to be a great option for couples who cannot conceive naturally. However, there is a small portion of couples who face difficulties in conceiving through a 3rd day embryo transfer through IVF. Some of these couples do not see results until they go through multiple cycles of IVF, while some do not see results at all. For these couples, a great alternative is to opt for a blastocyst IVF transfer on day 5 instead of fertilization instead.What is a blastocyst? A blastocyst is an embryo transfer which involves transferring one or more embryos that are at blastocyst formation stage of development which advanced stage of an embryo that usually happens on the 4th or 5th day of fertilization. This is then transferred to the uterus of the expecting female. To understand it better, it is important to understand the basic differences between a 3rd, 4th, and 5th-day transfer of an embryo.
3rd-Day Transfer
An embryo starts off as a single cell that divides every 12-24 hours. By the third day of fertilisation, the embryo has divided into 4 or 8 cells and is called a multicellular embryo. This is the stage where most natural IVF Cycles are performed. A 3rd Day embryo transfer is the most commonly used practice in IVF and usually has a high success rate if all the conditions are in the favour of the patient.
4th Day Transfer
Around the time of the 4 day, the embryo has divided into 16 cells and has arrived at the stage of what is medically known as Morula. Transfering the morula as part of an IVF Cycle is an unconventional method, though not totally unheard of. There is little scientific evidence that suggests that 4th day Morula transfers are in any way more or less successful than embryo transfer on the 3rd day.
5th Day Transfer
A blastocyst is an advanced stage of the embryo where the cell has differentiated into 2 different cell types, with more than 70-100 cells between them. The first one is the inner cell mass which will then turn into the tissues of the baby as it develops, while the other is the trophoblast or trophoectoderm, which forms part of the placenta. This cell division is the major reason why blastocyst transfer on the 5th-6th day are more successful than embryo transfer on the 3rd day. This is especially true and helpful for couples who have been through IVF cycles to no avail or have other reasons behind their inability to conceive naturally.
Blastocyst Implantation FAQ’s
Because of the difference of environment in the fallopian tube and the uterus, the blastocyst are more equipped to survive than the embryos. As a result, blastocyst transfer are as much as 80% successful in helping infertile couples.
After transfer, the blastocyst hatches out of its shell and attaches itself to the uterus by the end of day 2. Then the blastocyst begins to attach itself deeper into the uterine lining, beginning the implantation which continues till day 5th. The fetal and placenta tissue begin to develop, releasing the Human chorionic gonadotropin (hCG) into the blood stream. After the 9th Day, the hCG levels in the blood get to a level enough to detect confirmed pregnancy.
About 5 days after the transfer, the blastocyst completely implants itself to the uteral lining.
There is no evidence that suggests that a certain sleeping position is better for implantation than others. The blastocyst is transferred on the right time and when the womb is ready for it. What matters is that the expecting mother should be comfortable.
A 5th day blastocyst transfer is more successful than a 3rd day embryo transfer. For more information, get in touch with our experts by booking an appointment now.
The Don’ts of blastocyst transfer include:
- No alcohol
- No smoking
- No caffeine
- No fizzy drinks
- Avoid heavy lifting and strenuous activities
- Avoid hot baths
Our Doctors

Dr. Gauri Agarwal
Infertility Specialist
Dr. Gauri Agarwal is a young, dynamic and leading fertility specialist of Delhi/NCR. Dr. Agarwal is backed by a visionary thinking, die-hard determination and industry-rich experience of more than 10 years. She is the Director, IVF at Seeds of Innocence Centre. Dr. Agarwal finished her MBBS degree from Bharati Vidyapeeth Medical College, Pune. Post that, she did a Diplomate of National Board (DNB), Obstetrics and Gynaecology Residency Program from KG Superspecialty Hospital, Coimbatore. She followed this with a hard-earned Fellowship Program in Reproductive Medicine (Infertility) from the University of Gent, Belgium. Further, she received training in Embryology at the National University of Singapore.

Dr. Julie Chhawchharia
Infertility Specialist
Dr. Julie Chhawchharia, is a competent and committed Infertility specialist in Reproductive Medicine with around a decade of extensive experience along with experience in obstetrics and gynaecology. She has been performing vaginal deliveries, caesarean sections, and various gynaecological surgeries.. She is an acknowledged expert in Reproductive Medicine services by DY Patil University, Navi Mumbai. She is also a meritorious and Gold award-winning student of Fellowship and Diploma in Minimal Access Surgery (Laparoscopy) from the prestigious World Laparoscopy Hospital, Gurgaon in 2014. She completed her MBBS degree and MS in Obstetrics and Gynaecology from the renowned Institute JJM Medical College in Davangere, Karnataka.

Dr. Seema Madan
Infertility Specialist
Dr. Seema has done her M.D (Obstetrics & gynecology) from Maharshi Dayanand University, Rohtak Haryana. She has 19 year experience in Gynecology. Before joining Seeds of Innocence she was at ART at KJIVF and Laparoscopy Center as Consultant. She also worked at Fortis Bloom IVF, lady Harding Medical College, and Fortis Escorts Hospital & Research Centre.

Dr. Kriti Tiwari
Clinical Centre head
Dr. Kriti Tiwari is a qualified medical professional working as Clinical Centre head at Seeds Of Innocence, Malviya Nagar with more than 5 years of experience in infertility workup and management. She has completed her MBBS, M.S. Obstt. & Gynaecology, senior residency and FNB reproductive medicine all from one of the prestigious medical college of India, Maulana Azad Medical college, Delhi. Her key functioning areas in the field of infertility includes counselling and handling OPDs of patients with strong diagnostic skills, IUI, IVF, ICSI Procedures, IVF-embryo transfer, andrology, surgical sperm retrieval by PESA, TESA, laparoscopy & hysteroscopy and obstetric management of patients. Dr. Kriti Tiwari is conscious for improving the quality of life of patients through accurate diagnosis, timely intervention and ethical treatment by expertise in managing difficult cases like multiple IVF failures, severe endometriosis, poor responders and severe male factor with high success rates. She has always been updated in the education of her relevant field by training many junior doctors under her, attending several conferences in her field. She has published various papers and chapters in renowned books and made contribution in this field. Her recent publication was a chapter on “conservative management of ectopic pregnancy” in Manual of ART and laboratory Sciences, 2022.

Dr Hina Ali
Infertility Specialist
Dr. Hina Ali, is a highly qualified and accomplished medical professional with a specialization in Reproductive Medicine. Dr. Ali holds an MBBS degree from the prestigious Maulana Azad Medical College in Delhi, India, and completed her MS from UCMS & GTBH, Delhi. With a remarkable track record, she was awarded the Gold Medal for her FRM fellowship. Dr. Ali has over ten years of experience in the medical field, and her expertise in IVF spans over seven years. With her vast knowledge and skillset, Dr. Hina Ali is dedicated to providing the highest quality care to her patients.

Dr. Mangla
Obstetrics and Gynaecology
Dr. Mangla is a highly qualified professional, with an M.B.B.S. degree from Gandhi Medical College, Bhopal, Madhya Pradesh in 1989, and an M.D. in Obstetrics and Gynaecology from the same institution in 1993. She has also completed fellowships in fetal Sonography & Infertility from prestigious organizations. With a pioneering spirit, she established the first IVF center in Bhopal and has since gained more than 25+ years of vast experience. Dr Mangla is an astute professional with rich and extensive experience in Interventional Sonography. Her expertise extends to all facets of clinical practice, including patient care, research, and academics. She has also been a key member of the organizing committee for numerous national and international conferences focused on ART procedures.

Dr. Nivedita
Infertility Specialist
Dr. Nivedita is a highly qualified medical professional, holding an M.B.B.S. degree from Era Medical College in Lucknow, Uttar Pradesh, India. She completed her Compulsory Rotating Internship training from 2013 to 2014, further demonstrating her commitment to excellence in the medical field. She has also earned an M.S. in Obstetrics and Gynaecology from D.Y. Patil Medical College in Kolhapur, Maharashtra, India, showcasing her dedication to specialization in this area of medicine. In addition to her formal education, Dr. Nivedita has undergone rigorous training and achieved fellowships in fetal Sonography, Infertility, and Hysteroscopy from renowned organizations. Her extensive education and training have equipped Dr. Nivedita with the knowledge, skills, and expertise to provide her patients with exceptional medical care. Her commitment to professional development and her dedication to staying current with the latest medical advancements make her a highly respected and trusted medical practitioner in her field.